Title: Systematic-Review: Fertilizing capacity of rat spermatozoa is correlated with decline in straight-line velocity measured by continuous computer-aided sperm analysis
In this particular study, rat spermatozoa were recovered from various regions of the excurrent ducts of 10 adult males consisting of the Distal Cauda (DC) Proximal Cauda (PC) epididymitis as well as the Vas deferens (VD). With the use of limited sperm numbers, the ten male adults were assessed by Vitro fertilization. They were also analyzed through the continuous evaluation of the parameters of motility in an incubation period of 5 hours in vitro using the automated Computer-Aided Sperm Analysis (CASA). The results of this particular study showed that the in vitro fertilizing capacity of rat spermatozoa had a direct correlation with various things. Firstly, it was directly related to the decline in straight-line velocity (VSL) and secondly, related to the site of recovery mature rat spermatozoa which was derived from the distal excurrent duct.
A myriad of scholars contend that there is a significant relation between male fertility and the exposure to occupational and environmental factors. For this reason, the rat has been used as the primary animal model that is valid for assessing reproductive toxicology in males. Precisely, according to Vouk & Sheehan (1983), in the modern day today, the multi-generation, better known as the single breeding trial remains the most efficient method for the study of fertility. With reference to the indication of sperm fertilizing capacity, the measurements of the sperm motility are considered significant. (1) In a similar regard, Computer Aided Sperm Analysis is a standard method that has, in the past couple of decades been regarded as an effective method for quantitative analysis of rat sperm motility. From a general point of view, computer-aided sperm analysis involves an image oriented analysis that gives room to movement parameters of approximately several hundreds of spermatozoa in approximated time intervals of about 0.5 to 3 seconds. (2) This being the case, this particular investigation seeks to examine the existing relationship between the change in the parameters of motility in the incubation for rat spermatozoa which was recovered from different regions of both the vas deferens and the distal epididymis with an outcome of the In Vitro Fertilization (IVF).
PICO Question: Is there a relationship between the fertilizing capacity of Rat Spermatozoa with the decline of straight-line velocity, when measured using the continuous Computer Aided Sperm Analysis?
Participants: Rats, the Sprague-Dawley strain, Hull, Bantam, U.K., and Kingdom
Interventions: Computer-aided sperm analysis of the fertilizing capacity of rat spermatozoa.
Comparisons: Without the use of computer-aided sperm analysis.
Outcome: The relationship between the fertilizing capacity of Rat Spermatozoa with the decline of straight-line velocity
The Eligibility Criteria of The Study
The primary participants of this particular study were rats, which for them to be eligible for the study, had to be maintained on a light regime for approximately 14 hours of light and another 10 hours of dark. Besides, for all the rats to be eligible for this particular study, they were all fed on a specific rat diet.
For the collection of the sample spermatozoa to be used in the study, adult male rats with an approximate weight of about 475-650 grams were killed after which the excurrent duct and the testis were recovered through a midline incision. For the purposes of the comparison of the fertilizing capacity of the spermatozoa derived from each of the three studied duct segments, three additional experiments were carried out with the use of the pooled samples. The pooled sperm samples, both from the right and the left were then put together in the form of a stock suspension for more dilution for IVF procedure.
For this particular systemic review, a preliminary analysis that made a comparison of the spermatozoas fertilizing capacity from the proximal cauda, vas deferens and the distal cauda region of every excurrent duct of 10 male rats was done. Besides, the mean length of time that sperm was tracked in the study was in the range of 1-7 seconds. (3) Thus, based on this context, there was no particular difference in track numbers and samples which were derived from different regions of the tract. Also, in the incubation period, the spermatozoa from the DC or VD indicated a decline in straight-line velocity (VSL) as compared to the spermatozoa that were collected from the PC. Therefore, based on the total results of the study, it was noted that there was no significant correlation between the VSL which was initially collected and the fertilizing capacity.
Discussions and Findings
Based on the results of this particular study, it was found that there is an overall increasing awareness of the heterogeneous nature of what was considered as normal spermatozoa in a particular population. In a similar regard, various features of the Computer Aided Sperm Analysis (CASA) were considered to be crucial factors in the process of attaining a valid and an accurate analysis of the movement of rat spermatozoa. For this reason, there was an established correlation in decline in straight line velocity it the course of time, with the fertilization outcome in vitro.
Moore, H. D., & Hartman, T. D. (1969). In-vitro development of the fertilizing ability of hamster epididymal spermatozoa after co-culture with epithelium from the proximal cauda epididymidis. Reproduction, 78(2), 347-352. doi:10.1530/jrf.0.0780347
Chan, S.Y.W., and L.C.H. Tang. "Effect Of Reserpine On Fertilizing Capacity Of Human Spermatozoa". Contraception 30.4 (1984): 363-369. Web.
Taylor, T.H. et al. "Computer-Aided Sperm Analysis (CASA) Parameters And Its Relationship To Conventional In-Vitro Fertilization Rates". Fertility and Sterility 90 (2008): S453. Web.
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